10427 Abpz Status: Cancelled
| Gene | Abpz |
| Chromosome | 7 |
| Deletion Start | 33,739,282 |
| Deletion End | 33,737,305 |
| Deletion Size | 1,978 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,739,246 | 33,739,265 | GCTGGCCTTGCTGATGATTG |
| TUP | 33,739,215 | 33,739,242 | AGCTGGGCTTCCATACAAGTGAGAGTGG |
| TUR | 33,739,155 | 33,739,177 | GGTCTGTCTCATGCCACACTTAG |
| TDF | 33,738,249 | 33,738,268 | TGCAGGTTCCCACAATTCAC |
| TDP | 33,738,220 | 33,738,247 | CCCACATGCTGGGTAAACATAGGGTTCC |
| TDR | 33,738,169 | 33,738,190 | CATGCCTGTGTAGAGGCCATAC |
PCR Primers
| Primer | Sequence |
| SU | CCATACTCCTGCCTCTATGC |
| SD | AAGGCTGCAATGGCTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 314 bp | - | + | + |
| TUF | TUR | 111 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 115515 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
