10429 2510039O18Rik Status: ES cell colonies screened / QC no positives
| Gene | 2510039O18Rik |
| Chromosome | 4 |
| Deletion Start | 147,941,028 |
| Deletion End | 147,946,650 |
| Deletion Size | 5,623 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,941,736 | 147,941,757 | AAGGTCGGAGATCACCAGTTTC |
| TUP | 147,941,761 | 147,941,781 | TCTACTCGGGCCGGTCCACAC |
| TUR | 147,941,825 | 147,941,845 | TGGAGCCTGTTCACTAACTTC |
| TDF | 147,946,293 | 147,946,313 | CCAGCCAACATGCCACATCAG |
| TDP | 147,946,322 | 147,946,344 | CCTGGGCCAGGAGCTGTCTGTTT |
| TDR | 147,946,367 | 147,946,386 | GACAGGGAAGCAGACCACAG |
PCR Primers
| Primer | Sequence |
| SU | AGACTCAGCGACGCCTTAC |
| SD | CTGGGATAGAAGAAGAAATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 312 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 343 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
