10433 Clcnkb Status: ES cell colonies screened / QC no positives
| Gene | Clcnkb |
| Chromosome | 4 |
| Deletion Start | 141,415,292 |
| Deletion End | 141,404,520 |
| Deletion Size | 10,773 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,414,720 | 141,414,739 | CACACGTACCTGCCACAAAC |
| TUP | 141,414,690 | 141,414,717 | TGCTCGATCCTCATGTAAGGCAGAGTCC |
| TUR | 141,414,641 | 141,414,659 | GGCCAGACAGGGTAGAGAC |
| TDF | 141,404,805 | 141,404,825 | TGAGCTGCTGAACCTTCAGAC |
| TDP | 141,404,768 | 141,404,790 | CACGAGGCAGAGCTGTGGGCTCT |
| TDR | 141,404,746 | 141,404,767 | CCAGTACCTCCACCCACGATAC |
PCR Primers
| Primer | Sequence |
| SU | CCATGAGTCATGTGAGTGAG |
| SD | TACTCTGCACACCCTTAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
