10434 Tmem239 Status: ES cell colonies screened / QC positives
| Gene | Tmem239 |
| Chromosome | 2 |
| Deletion Start | 130,406,727 |
| Deletion End | 130,407,311 |
| Deletion Size | 585 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,406,798 | 130,406,818 | CTGGATCATCCGTCAGGACTG |
| TUP | 130,406,821 | 130,406,842 | TGCGCTGGTGGGTATGCCACAT |
| TUR | 130,406,861 | 130,406,881 | CAGCCTGATGTATTCCACCAC |
| TDF | 130,407,128 | 130,407,147 | CCTGCTCACCTCCATGACTC |
| TDP | 130,407,149 | 130,407,174 | ACCAGGCTACGCTCAGGACTTGGACC |
| TDR | 130,407,201 | 130,407,221 | CCAGGGCTCAACAGACACAAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGACACAACAGGAAGCAC |
| SD | GATCCGGGACAAATCCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 329 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
