10436 Hdhd3 Status: Cancelled
| Gene | Hdhd3 |
| Chromosome | 4 |
| Deletion Start | 62,499,934 |
| Deletion End | 62,499,162 |
| Deletion Size | 773 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,499,758 | 62,499,777 | GGGCTCAGAGCCACAACTTC |
| TUP | 62,499,734 | 62,499,756 | CCAACTATGGCCTGAGCCGTGGC |
| TUR | 62,499,701 | 62,499,721 | GACCACGTCCTTCCACCATTG |
| TDF | 62,499,512 | 62,499,534 | CCAACTTTGACCGACGACTAGAG |
| TDP | 62,499,486 | 62,499,508 | ATCCTAACAGGCCTTGGCCTGCG |
| TDR | 62,499,426 | 62,499,444 | CGTGGGTCGGGTTTAGGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGCATCTACCTCACCTACC |
| SD | GGGATGAGTAGTGAGCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 307 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 260 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
