10437 Qrich2 Status: ES cell clone microinjected
| Gene | Qrich2 |
| Chromosome | 11 |
| Deletion Start | 116,453,580 |
| Deletion End | 116,441,088 |
| Deletion Size | 12,493 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,453,224 | 116,453,244 | GCATCCAAAGCAGTGCAGAAC |
| TUP | 116,453,200 | 116,453,221 | TGGGAGCACTTGACTGCCGCTC |
| TUR | 116,453,147 | 116,453,167 | GGGAGGAGCCTGTATTTCAAC |
| TDF | 116,441,748 | 116,441,769 | GAGGCATAAATGAGGCAGGTTC |
| TDP | 116,441,721 | 116,441,745 | TCTGCAAGGATCACGGAAGGGCCAT |
| TDR | 116,441,688 | 116,441,711 | CAAGCATTCGTGGGTGTTCCTAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGGAGGAACGCCAGTGTC |
| SD | GAACCAGGTCTGAAGTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 299 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
