| Gene | Tlr13 |
| Chromosome | X |
| Deletion Start | 106,147,581 |
| Deletion End | 106,159,722 |
| Deletion Size | 12,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,148,090 | 106,148,112 | GAAGCTATCCAGTCATGGTAGAC |
| TUP | 106,148,125 | 106,148,153 | TAGCAGGGTTTACACACAGATACCCTCAC |
| TUR | 106,148,157 | 106,148,180 | TTCCTTTCACTGAAGCACTAACTC |
| TDF | 106,158,857 | 106,158,881 | CACCTGTGACAATTTGTGGTTCAAG |
| TDP | 106,158,885 | 106,158,910 | TGGTCAATGAACACAGAGGAGGTCCA |
| TDR | 106,158,929 | 106,158,948 | CTGGCTGCTGACAGGGATAG |
PCR Primers
| Primer | Sequence |
| SU | GTTGAGAGCCAAGGGAGAAG |
| SD | GTCTCCCATGACCACAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 343 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10438A-F6 | VGB6 | C57BL/6NTac | |
| 10438B-B6 | VGB6 | C57BL/6NTac | |
| 10438B-C7 | VGB6 | C57BL/6NTac | |
