10440 Plek Status: Cancelled
| Gene | Plek |
| Chromosome | 11 |
| Deletion Start | 17,008,595 |
| Deletion End | 16,981,802 |
| Deletion Size | 26,815 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,008,322 | 17,008,340 | AGTTCGGGCTTCAGCACAG |
| TUP | 17,008,295 | 17,008,319 | TGCCCTTACGAGCCTGAGAATTCGA |
| TUR | 17,008,273 | 17,008,293 | TCATCGTCCACACCCTGAATG |
| TDF | 16,982,774 | 16,982,793 | GGATCTCAGGTCCTGCTCTG |
| TDP | 16,982,735 | 16,982,762 | CTGCACTAGATGCCATTGATTGACGCGT |
| TDR | 16,982,657 | 16,982,676 | GGCTGCAAATGCCACATCTC |
PCR Primers
| Primer | Sequence |
| SU | GGAAAGCATGGGCTGTTCAG |
| SD | GAAAGGCTATGTAGCCTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 325 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
