10443 Med22 Status: Cancelled
| Gene | Med22 |
| Chromosome | 2 |
| Deletion Start | 26,910,412 |
| Deletion End | 26,905,732 |
| Deletion Size | 4,681 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,909,890 | 26,909,912 | CCTTTGAGTCGTAAATGCCAGTG |
| TUP | 26,909,864 | 26,909,885 | CCGATGGTCTTAGGCAGCCCCT |
| TUR | 26,909,842 | 26,909,863 | CAACCTGTGGATTGCCTTTCAC |
| TDF | 26,906,337 | 26,906,356 | GTTAGGCCAGGACCCTGTAG |
| TDP | 26,906,311 | 26,906,333 | TGGAAACAGGTCTGCCACCAGGA |
| TDR | 26,906,277 | 26,906,298 | ACCAGTAGGGTAGTGATGAGAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTTCCCTCGCCGTAAGG |
| SD | CTAAACAAACTGAGGCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 249 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
