10445 Coasy Status: Cancelled
| Gene | Coasy |
| Chromosome | 11 |
| Deletion Start | 101,082,995 |
| Deletion End | 101,086,328 |
| Deletion Size | 3,334 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,083,203 | 101,083,225 | TGGATTTCATCACGCACCTCTAC |
| TUP | 101,083,226 | 101,083,247 | ACTGGTGCCGACCTGCACAGGC |
| TUR | 101,083,268 | 101,083,291 | GCTCTTGGTTTGGATATTGGTCAG |
| TDF | 101,085,635 | 101,085,652 | TGACGGGAGCAGCCAAAG |
| TDP | 101,085,653 | 101,085,675 | TGGGACAGTTAAGCCGCAACACC |
| TDR | 101,085,676 | 101,085,695 | GGCCAAGGAAGATCCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GGGCTGGGATTCCTGAAGC |
| SD | AGAACCTCAGAGCGGATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 319 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 61 bp | + | + | - |
| NeoFwd | SD | 279 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
