10448 Ifne Status: Cancelled
| Gene | Ifne |
| Chromosome | 4 |
| Deletion Start | 88,880,176 |
| Deletion End | 88,879,580 |
| Deletion Size | 597 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,880,133 | 88,880,152 | GTGTTGCTGCTCTTGGTTTC |
| TUP | 88,880,102 | 88,880,131 | TCCACTATCTTCTCCCTAGAACCGAAACGG |
| TUR | 88,880,045 | 88,880,068 | TGGCAAAGGTTTCAGTAGTTGTAG |
| TDF | 88,879,679 | 88,879,698 | GCTGTGCCTGGATCATTGTC |
| TDP | 88,879,640 | 88,879,668 | TCCACCGCTGTATGTTCTTTGTGTTCAGG |
| TDR | 88,879,618 | 88,879,637 | GTCTGCTCAGCCATGTTGTG |
PCR Primers
| Primer | Sequence |
| SU | AGACAAGTGCACAGTGAAGC |
| SD | GAAAACATAACATGGCATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
