10449 Gpr135 Status: Cancelled
| Gene | Gpr135 |
| Chromosome | 12 |
| Deletion Start | 72,070,988 |
| Deletion End | 72,069,585 |
| Deletion Size | 1,404 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,070,525 | 72,070,543 | GCCGCTTCTTCAGTTCGTG |
| TUP | 72,070,498 | 72,070,522 | TCGGAATCGTATCCACGTTCAGCGT |
| TUR | 72,070,472 | 72,070,493 | AGTAGCGGTCCAGCGAGATGAG |
| TDF | 72,069,818 | 72,069,837 | TCAAGCCAGAAGTCGCAATC |
| TDP | 72,069,790 | 72,069,815 | CTTCGAAATGGCTGTGCCAACAGGCT |
| TDR | 72,069,748 | 72,069,769 | CCACTAGCTGGGTTGGAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTGGGGTACACCGCTAG |
| SD | CGCATTTGAAGAAAGGGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 319 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 257 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
