10450   Mup3     Status: ES cell colonies screened / QC no positives

GeneMup3
Chromosome4
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:97235
NCBI Gene ID17842
BAC ID245D21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF62,086,35062,086,371CAGGCAACATTACAGAGTGCTG
TUP62,086,32362,086,347TTAAGGCAGATGGTGGCTATCTGGC
TUR62,086,28162,086,303GTTTCACCATTGCTGTGCTTCAG
TDF62,085,11762,085,135GGTCTTCCCAGCTTGGTCA
TDP62,085,08962,085,116TGCCACTTTCTCACCTCCTGAAGATTGG
TDR62,085,00062,085,028GATTTGGCATCCATAGAATATCCTTCTAC

PCR Primers

PrimerSequence
SUATCCATAGGTGATGAGATTGC
SDGGCCTAAGTGCTTGGGTCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR91 bp++-
TDFTDR136 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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