10453 Tcap Status: Cancelled
| Gene | Tcap |
| Chromosome | 11 |
| Deletion Start | 98,383,830 |
| Deletion End | 98,384,546 |
| Deletion Size | 717 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,384,115 | 98,384,135 | CCTCAGTTCACCACCTTTGTC |
| TUP | 98,384,136 | 98,384,163 | TCCTTTCCACTTTCAGATGCTCCTTGCA |
| TUR | 98,384,171 | 98,384,192 | AGGTCTCATGCCTCTGTGTATC |
| TDF | 98,384,242 | 98,384,260 | GGTGATGCGCCTGGGTATC |
| TDP | 98,384,262 | 98,384,284 | TCGGCCGTGGGCTACAGGAATAC |
| TDR | 98,384,317 | 98,384,336 | CCTTGGTGGGCGTGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | GCAACCAGAATCTGGGTACC |
| SD | CAGCTCTCCACTCCTAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 311 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 295 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
