10455 Prl7c1 Status: Cancelled
| Gene | Prl7c1 |
| Chromosome | 13 |
| Deletion Start | 27,780,746 |
| Deletion End | 27,773,598 |
| Deletion Size | 7,149 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,780,552 | 27,780,574 | GAAGGCCAGATGGTACTGAGAAG |
| TUP | 27,780,528 | 27,780,547 | TTCTAAGCCTTACTCTAGAA |
| TUR | 27,780,496 | 27,780,519 | CAGATATAAGGGCCTACTGTGGAG |
| TDF | 27,774,509 | 27,774,536 | TAGAGACTATCCTGATGATACTCAGCAG |
| TDP | 27,774,472 | 27,774,501 | TCTCTTCCTGCCTTCCACTTTACTTCATCA |
| TDR | 27,774,382 | 27,774,408 | AGCTGCATGTAAGATGGAATGTCTACA |
PCR Primers
| Primer | Sequence |
| SU | ATGGAGAAGAGACTACCTTAG |
| SD | GTGCCACTTTGAGGTCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 347 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 155 bp | + | + | - |
| NeoFwd | SD | 312 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
