| Gene | Gpr152 |
| Chromosome | 19 |
| Deletion Start | 4,142,447 |
| Deletion End | 4,144,033 |
| Deletion Size | 1,587 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,142,950 | 4,142,968 | TGTGCCCTGGTTGGTCTTC |
| TUP | 4,142,976 | 4,143,002 | CTGCTGTCTGGTGGTATGACCTGGTCA |
| TUR | 4,143,020 | 4,143,039 | AGAGGCAGCTCCTCGGTGTC |
| TDF | 4,143,378 | 4,143,396 | TGCTGCGAACTGTGCTCTC |
| TDP | 4,143,398 | 4,143,418 | TCCTTTGCGGCTGCTGTCTGC |
| TDR | 4,143,439 | 4,143,457 | CTGTGGCTCGGCAGGTATG |
PCR Primers
| Primer | Sequence |
| SU | GTAACCTTAACCAAATCACAGAG |
| SD | CCAGCAAGACCATCCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10458B-A8 | VGB6 | C57BL/6NTac | |
| 10458B-D4 | VGB6 | C57BL/6NTac | |
| 10458B-E7 | VGB6 | C57BL/6NTac | |
