10462 Tshb Status: ES cell colonies screened / QC no positives
| Gene | Tshb |
| Chromosome | 3 |
| Deletion Start | 102,778,308 |
| Deletion End | 102,777,469 |
| Deletion Size | 840 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,778,189 | 102,778,207 | GTGTGCCTACTGCCTGACC |
| TUP | 102,778,163 | 102,778,187 | TCAACACCACCATCTGTGCTGGGTA |
| TUR | 102,778,134 | 102,778,156 | GCATTGAACAACATACCCGTGTC |
| TDF | 102,777,600 | 102,777,624 | CGCACCATGTTACTCCTTATTTCTC |
| TDP | 102,777,576 | 102,777,598 | TTCCCTGTCGCCATAAGCTGCAA |
| TDR | 102,777,521 | 102,777,542 | GGTTCTGACAGCCTCGTGTATG |
PCR Primers
| Primer | Sequence |
| SU | GGTCGTAGCTGCATCTGATC |
| SD | GTGTGGAAACCATGACAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | 273 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
