10464 Aoc2 Status: Cancelled
| Gene | Aoc2 |
| Chromosome | 11 |
| Deletion Start | 101,325,077 |
| Deletion End | 101,329,464 |
| Deletion Size | 4,388 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,325,136 | 101,325,159 | TCACTGTCTTTGCTCTGGTCTATG |
| TUP | 101,325,161 | 101,325,180 | CCTGCTGACCAGGCAAGGCA |
| TUR | 101,325,181 | 101,325,200 | TCGAGGAGACTGGCTGAAAC |
| TDF | 101,329,005 | 101,329,024 | GTCCGGCCTCTGTCCAATAC |
| TDP | 101,329,030 | 101,329,059 | ATCCCTACTAGGCAGAAATTAGTCACAGCA |
| TDR | 101,329,091 | 101,329,110 | TGCCAGCCACATTCCTACAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGGAGCAAGCTATGTAATG |
| SD | CAGAGGAGTGTGTACATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 317 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 341 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
