10469 Arhgef19 Status: ES cell colonies screened / QC no positives
| Gene | Arhgef19 |
| Chromosome | 4 |
| Deletion Start | 141,246,186 |
| Deletion End | 141,256,918 |
| Deletion Size | 10,733 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,246,800 | 141,246,822 | TGTCAGGCCCTCTAGTCTCTATG |
| TUP | 141,246,824 | 141,246,850 | AAAGCTCTTGCGATGATCTCATTGCCC |
| TUR | 141,246,866 | 141,246,885 | GCGGCATGACCCTGAACATC |
| TDF | 141,256,372 | 141,256,394 | ACAGACATCCTGGCAGTGAAGAC |
| TDP | 141,256,395 | 141,256,418 | CAGGACCAGCGATGGTGAGAACCA |
| TDR | 141,256,456 | 141,256,479 | CCCTGAGACAGGTTGGTCATGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGGGTGCTTCCTCATCTG |
| SD | CAAGCCTGCTTGGATCTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 327 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
