10472   Krtap4-7     Status: ES cell colonies screened / QC no positives

GeneKrtap4-7
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1923694
NCBI Gene ID76444
BAC ID102E13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF99,643,98499,644,002GAGGAGGGCTGTAGCCAAG
TUP99,643,95999,643,979CTGCCAGCCCAGCTGCTGTGT
TUR99,643,92199,643,941ACACAGACTGGCAGCACTGAG
TDF99,643,69799,643,717CCATCTTGTGGTGGTTCTAGC
TDP99,643,66899,643,688CTCCAGCTGCTGCCGCCCTTG
TDR99,643,56499,643,583GGCAGGTGGAGATGACACAA

PCR Primers

PrimerSequence
SUTCAGTTTAGAAGGTACATAGGCA
SDTGAGGACCCTTGAGAGTTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR82 bp++-
TDFTDR154 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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