10473 Nudt10 Status: Cancelled
| Gene | Nudt10 |
| Chromosome | X |
| Deletion Start | 6,172,832 |
| Deletion End | 6,172,342 |
| Deletion Size | 491 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,047,925 | 6,047,942 | GAACCAGACGCGGACCTA |
| TUP | 6,047,943 | 6,047,965 | CGACCCCGAGGGCTTCAAGAAGC |
| TUR | 6,048,133 | 6,048,150 | CAGCAAGCGGCCCAACTT |
| TDF | 6,048,211 | 6,048,230 | ACCGAGCTGCTGGAGGATTG |
| TDP | 6,048,234 | 6,048,257 | AGACTCGGTCAGCATCGGCAGGAA |
| TDR | 6,048,267 | 6,048,289 | TGATGGCATCTTCGATCTTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGATCGCGGCGATCCGGAG |
| SD | CCATGCAGCTGTGGCTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 541 bp | - | + | + |
| TUF | TUR | 226 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 374 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
