10474 Gpr68 Status: Cancelled
| Gene | Gpr68 |
| Chromosome | 12 |
| Deletion Start | 100,879,280 |
| Deletion End | 100,878,186 |
| Deletion Size | 1,114 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,879,110 | 100,879,128 | CCGGAATGAGCTGGGAGTG |
| TUP | 100,879,082 | 100,879,107 | CCTGTGTAACCTGACCATTGCAGACC |
| TUR | 100,879,048 | 100,879,066 | TGCAGCCAGAAGGGAAGTG |
| TDF | 100,878,603 | 100,878,623 | AGCCGCAAGGACCAGATTCAG |
| TDP | 100,878,583 | 100,878,602 | CGGCTGGTGCTCAGCACCGT |
| TDR | 100,878,560 | 100,878,581 | GAAAGCAAGCCAGGAAGATGAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTTGTACCAGGGGGCTAG |
| SD | AACATGGGAGGAGACAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 476 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
