10477 Tprgl Status: Cancelled
| Gene | Tprgl |
| Chromosome | 4 |
| Deletion Start | 154,160,626 |
| Deletion End | 154,158,336 |
| Deletion Size | 2,291 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,160,183 | 154,160,207 | GCTGCTCATCTGCAAATATGACTTC |
| TUP | 154,160,159 | 154,160,181 | TCAGCCTTCAGTGCCAGCAGGTG |
| TUR | 154,160,090 | 154,160,108 | GAGCGACTTCGGAGGAAAC |
| TDF | 154,159,264 | 154,159,285 | CAGCGTCTCTGTGCCATGTAAG |
| TDP | 154,159,241 | 154,159,263 | CAGGCCAAGGCCACTGTCAGTGT |
| TDR | 154,159,196 | 154,159,213 | CAGCACCACCTAGCTTCC |
PCR Primers
| Primer | Sequence |
| SU | CTTCCTGTAAACAGGAAGTGG |
| SD | AGTGGGTGGACATTGTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 387 bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 312 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
