| Gene | Gsg2 |
| Chromosome | 11 |
| Deletion Start | 73,138,258 |
| Deletion End | 73,135,753 |
| Deletion Size | 2,506 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,137,397 | 73,137,417 | GCAAAGACCTTCGTGTACCAG |
| TUP | 73,137,365 | 73,137,391 | ATCAGCAGGCCTAAGAGAACTGGGCCA |
| TUR | 73,137,333 | 73,137,353 | CCCGTTGCTTCTTGCTGTTTC |
| TDF | 73,136,504 | 73,136,525 | GACTGCAAAGAGCATTCTCCAC |
| TDP | 73,136,477 | 73,136,499 | TCACTGCATCTCTGGCTGTGGCA |
| TDR | 73,136,445 | 73,136,464 | AAGTCCCGGTGCTCAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | ACACGCAGGCTCTTGACTC |
| SD | CTTGGTTTTGACTCTCTAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 274 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10478A-H4 | VGB6 | C57BL/6NTac | |
| 10478A-F8 | VGB6 | C57BL/6NTac | |
