10486 Tusc1 Status: Cancelled
| Gene | Tusc1 |
| Chromosome | 4 |
| Deletion Start | 93,335,417 |
| Deletion End | 93,334,784 |
| Deletion Size | 634 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,335,185 | 93,335,200 | AGCGGGACCGGCAGAA |
| TUP | 93,335,167 | 93,335,182 | CGCGGCTGCGCGAGGA |
| TUR | 93,335,149 | 93,335,165 | AGCCTCAGGCGGGCGTT |
| TDF | 93,335,026 | 93,335,041 | ACGAGCCCGCCACCAA |
| TDP | 93,335,006 | 93,335,025 | CCGCAAGGCGAGAGGCCATG |
| TDR | 93,334,929 | 93,334,946 | CGCTCGGCGGTACATGAC |
PCR Primers
| Primer | Sequence |
| SU | CACCCAGCAGAGGACAAGGC |
| SD | AGAGTTGTTGGACCCCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 416 bp | - | + | + |
| TUF | TUR | 52 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
