10489 Ppm1n Status: Cancelled
| Gene | Ppm1n |
| Chromosome | 7 |
| Deletion Start | 19,280,021 |
| Deletion End | 19,277,198 |
| Deletion Size | 2,844 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,279,242 | 19,279,261 | GCCAGGATGAGGACGAGTTC |
| TUP | 19,279,217 | 19,279,240 | TTCTCCTGGCTTCTGATGGCGTGT |
| TUR | 19,279,188 | 19,279,206 | CGCCAGATCAGCACCAGAC |
| TDF | 19,277,910 | 19,277,930 | ATCTTTGCCCACTCGCCTCAC |
| TDP | 19,277,875 | 19,277,899 | CCAGCGCTCTGGAGTGAGGAACTTA |
| TDR | 19,277,856 | 19,277,874 | AGCCTTCAACTGCCTTACC |
PCR Primers
| Primer | Sequence |
| SU | ATAGGAAAACTGACTCTCAC |
| SD | GGTTTAGTTGGGTTTTCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 499 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
