10497 Kcnb1 Status: Cancelled
| Gene | Kcnb1 |
| Chromosome | 2 |
| Deletion Start | 167,188,620 |
| Deletion End | 167,188,067 |
| Deletion Size | 573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 167,188,420 | 167,188,438 | CTGCAACACGCACGACTCT |
| TUP | 167,188,397 | 167,188,418 | TGCTCCAGGTGTGCGACGACTA |
| TUR | 167,188,357 | 167,188,376 | AGGGTGGCGGTCGAAGAAGT |
| TDF | 167,188,204 | 167,188,223 | AGGCCCGCTACCACCAAAAG |
| TDP | 167,188,176 | 167,188,203 | AAGGAGCAGATGAACGAGGAGCTGAAGC |
| TDR | 167,188,086 | 167,188,105 | CCAGCAGGTCCCAAAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | ACAGCGGCCTGCAGAGCTCC |
| SD | CTGCCTTTTAACCGGTATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 223 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 138 bp | + | + | - |
| NeoFwd | SD | 195 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
