10499 Slfnl1 Status: Cancelled
| Gene | Slfnl1 |
| Chromosome | 4 |
| Deletion Start | 120,533,157 |
| Deletion End | 120,536,527 |
| Deletion Size | 3,371 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,533,629 | 120,533,650 | GCCCTGAAGAGCATGAAGAGAC |
| TUP | 120,533,653 | 120,533,680 | AAGAGTGGATAGCATGAGGAACTGCACA |
| TUR | 120,533,697 | 120,533,720 | TCTTAGCAGAGGGTCAGTCATTTG |
| TDF | 120,535,587 | 120,535,605 | GGCTGCCGTCAGGAGTATG |
| TDP | 120,535,610 | 120,535,637 | TACCCAAGTAGAACTGGGACTACCTCCA |
| TDR | 120,535,674 | 120,535,695 | ACCGTCAGGCGGAGTACCTTAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGCTCTTTCCTAGGCAC |
| SD | GCTTACAACGTAGTCTTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 405 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
