10502 Med8 Status: ES cell clone microinjected
| Gene | Med8 |
| Chromosome | 4 |
| Deletion Start | 118,409,365 |
| Deletion End | 118,414,789 |
| Deletion Size | 5,425 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,409,663 | 118,409,682 | TGGACACAGGCGTCATAAGG |
| TUP | 118,409,683 | 118,409,711 | CTTCAGAAGGCTAAAGGGATCGTGGGCCT |
| TUR | 118,409,732 | 118,409,753 | CCCACAGCCTCGAATTACAAAC |
| TDF | 118,413,897 | 118,413,915 | GCACAGGAGTGTGGGAATG |
| TDP | 118,413,923 | 118,413,949 | TCATCTAAGCCTCCTTCATCTGCACCC |
| TDR | 118,413,973 | 118,413,993 | CGAGGTGACAAAGCAAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | AGGATGGCATCTGAGTAAAC |
| SD | CCTCACAACAACCCTACGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 445 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
