10503 Gps2 Status: Cancelled
| Gene | Gps2 |
| Chromosome | 11 |
| Deletion Start | 69,914,343 |
| Deletion End | 69,916,505 |
| Deletion Size | 2,163 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,915,115 | 69,915,133 | TGACCTGTCTTCCTTCCAC |
| TUP | 69,915,134 | 69,915,162 | TCCAGTGACTTAACCACTCTGACATCAGC |
| TUR | 69,915,165 | 69,915,184 | CGTCAGGCTCTGCTGGTATG |
| TDF | 69,916,131 | 69,916,152 | GAGGCCAAGGTGCTAAGATGAG |
| TDP | 69,916,159 | 69,916,183 | CCCAGGATGCCTCCCTTTAAGGTCA |
| TDR | 69,916,185 | 69,916,209 | CCGCAGAGAAGACTAGGAGACAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGAAAGCGCCGACAAGGC |
| SD | ATATGAAGCCCCCAACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
