10504 Cd53 Status: Cancelled
| Gene | Cd53 |
| Chromosome | 3 |
| Deletion Start | 106,770,741 |
| Deletion End | 106,760,782 |
| Deletion Size | 9,960 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,770,511 | 106,770,532 | TTCCTCACTTTCTGCCATTGTG |
| TUP | 106,770,492 | 106,770,510 | TGACACCATAAGTCTACAA |
| TUR | 106,770,429 | 106,770,451 | CATCCCTAAATCCCCAGAGAGTC |
| TDF | 106,760,870 | 106,760,893 | TCTCAATTCTCTTCTCAGGTGCTG |
| TDP | 106,760,839 | 106,760,865 | TGTCCTTTGCACTGACACTCAACTGCC |
| TDR | 106,760,817 | 106,760,837 | GCCTGGCTTGTTTTGTCAATC |
PCR Primers
| Primer | Sequence |
| SU | GAACTTCAGGCCAATTTTCC |
| SD | ACACCCAGGTATGTTTCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 353 bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
