10505 Krtap9-1 Status: Cancelled
| Gene | Krtap9-1 |
| Chromosome | 11 |
| Deletion Start | 99,879,220 |
| Deletion End | 99,874,002 |
| Deletion Size | 5,219 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,879,301 | 99,879,320 | ACCACCTGCTGGAGACCAAG |
| TUP | 99,879,322 | 99,879,343 | TGTGTGACCAGCTGTTGCCAGC |
| TUR | 99,879,417 | 99,879,436 | TCACACAGCATGGCTGGAAG |
| TDF | 99,879,727 | 99,879,746 | GTGACCTGCACCAGGACTTG |
| TDP | 99,873,922 | 99,873,945 | CCAGCCAACATGTGTTTGTGTGCC |
| TDR | 99,873,978 | 99,873,998 | AACAGCCACAGGGCTCACAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGCAACTTCCTGCTGTG |
| SD | TTAAACTTAAGGGAATGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 6095 bp | - | + | + |
| TUF | TUR | 136 bp | + | + | - |
| TDF | TDR | 5769 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
