| Gene | Zc3h12b |
| Chromosome | X |
| Deletion Start | 95,899,099 |
| Deletion End | 95,927,970 |
| Deletion Size | 28,872 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,899,162 | 95,899,181 | CAGCCAAAGCAGGAGGACAG |
| TUP | 95,899,184 | 95,899,208 | CAGACCGAGGCAATGCTGATCCTGA |
| TUR | 95,899,242 | 95,899,261 | GGCCACTCTTACAGCTCATC |
| TDF | 95,927,775 | 95,927,798 | GAGGAATCCTTGGGTTGGAATGTG |
| TDP | 95,927,807 | 95,927,834 | CTAGGGAGCACATGATACCAGAGCATCA |
| TDR | 95,927,841 | 95,927,863 | TGTTGCAGAGGTTCTTGTATGTC |
PCR Primers
| Primer | Sequence |
| SU | GAACTATTGGAATTAATGAC |
| SD | GTCATTTGGTAGCATCAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 441 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 304 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10508C-A12 | VGB6 | C57BL/6NTac | |
| 10508C-B7 | VGB6 | C57BL/6NTac | |
| 10508C-G6 | VGB6 | C57BL/6NTac | |
