10510 Ccdc107 Status: Cancelled
| Gene | Ccdc107 |
| Chromosome | 4 |
| Deletion Start | 43,493,434 |
| Deletion End | 43,495,906 |
| Deletion Size | 2,489 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,494,097 | 43,494,114 | ATCGCGGCAGCAACAGAG |
| TUP | 43,494,117 | 43,494,140 | TCTAGTGGACCAGACACCAAGCCG |
| TUR | 43,494,162 | 43,494,186 | CAGTCAGTTCACATTTCAGGGTTTC |
| TDF | 43,495,776 | 43,495,795 | AACCTGGAGCCCAGAAACAC |
| TDP | 43,495,799 | 43,495,823 | ACCTAGCTCCTTCCTGGGAGGTGGA |
| TDR | 43,495,831 | 43,495,850 | TTGTGCCACCTTCTCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CCCGATGTAGGCGTGGTCTC |
| SD | TTTGCAAAAGCCTTCCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 231 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
