10512 Med31 Status: Cancelled
| Gene | Med31 |
| Chromosome | 11 |
| Deletion Start | 72,214,985 |
| Deletion End | 72,211,866 |
| Deletion Size | 3,120 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,214,762 | 72,214,780 | GCTCCTGGGTTGCTCAAAC |
| TUP | 72,214,737 | 72,214,760 | CCTACTCCAACCCTGGCTCTTGGG |
| TUR | 72,214,690 | 72,214,708 | GCACAGACAGTGGAAGGTC |
| TDF | 72,211,990 | 72,212,009 | CGCAGTGTGCGAAGTTCATC |
| TDP | 72,211,963 | 72,211,987 | CGAGCAGCAGATTCTGCACTGGCAG |
| TDR | 72,211,942 | 72,211,960 | CACCCGCTTCCGAGAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCACGTCACACAGGGAAAGG |
| SD | AAGGTTCCAGGGTACCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 411 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 388 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
