| Gene | St6galnac6 |
| Chromosome | 2 |
| Deletion Start | 32,599,764 |
| Deletion End | 32,619,529 |
| Deletion Size | 19,766 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,599,973 | 32,599,992 | AAGGGAGGCAGGTCCTACAG |
| TUP | 32,599,993 | 32,600,017 | ACACGGGTGGCTTAGGACTCCTTCA |
| TUR | 32,600,036 | 32,600,058 | AGACACCATAGAGACGCTCATAC |
| TDF | 32,618,792 | 32,618,815 | TGTTGGTAATGACTGGATGGAGAG |
| TDP | 32,618,824 | 32,618,847 | TGCTAGCTGTGTTCTGCCGAATGC |
| TDR | 32,618,863 | 32,618,886 | GGACTAATGAGGCAGGAGATGATG |
PCR Primers
| Primer | Sequence |
| SU | ATCTAGACTACTCCTTTCTG |
| SD | TGATAGACTATGCTCAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 357 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 346 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10516B-A3 | VGB6 | C57BL/6NTac | |
| 10516B-A4 | VGB6 | C57BL/6NTac | |
| 10516B-E9 | VGB6 | C57BL/6NTac | |
| 10516B-F3 | VGB6 | C57BL/6NTac | |
| 10516B-F5 | VGB6 | C57BL/6NTac | |
| 10516B-F2 | VGB6 | C57BL/6NTac | |
