10519 Gltpd1 Status: ES cell colonies screened / QC no positives
| Gene | Gltpd1 |
| Chromosome | 4 |
| Deletion Start | 155,867,231 |
| Deletion End | 155,866,337 |
| Deletion Size | 895 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,867,062 | 155,867,079 | TTGCCAGCCAGAGTCCAC |
| TUP | 155,867,038 | 155,867,060 | TGGGACCTCTGCATCCCGCATGG |
| TUR | 155,866,993 | 155,867,012 | GGCAGGAAAGCCAGATAGTC |
| TDF | 155,866,558 | 155,866,576 | CAATGCCACGCTGGCTAAC |
| TDP | 155,866,526 | 155,866,554 | CACTCCTGGATAGTACGTCAAGCTGTCAC |
| TDR | 155,866,499 | 155,866,519 | CGTGAGGGTAAGGCACAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CACAGCAGGGTCAAAGTGGC |
| SD | GCCTGAACACACACATAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 320 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
