10520 Hdgfl1 Status: ES cell colonies screened / QC positives
| Gene | Hdgfl1 |
| Chromosome | 13 |
| Deletion Start | 26,770,085 |
| Deletion End | 26,769,218 |
| Deletion Size | 868 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,769,824 | 26,769,843 | CGAGCACGACCCTATGGTTG |
| TUP | 26,769,801 | 26,769,823 | AGGCCTCCTCTAGCCTGTGCTCA |
| TUR | 26,769,778 | 26,769,800 | TCTGTGTAGCTCTGATCCTCTTC |
| TDF | 26,769,427 | 26,769,448 | CGGAGGATGAAGAGCCTCATTG |
| TDP | 26,769,405 | 26,769,426 | TCCTCTCAAACGGCCCAGGGAG |
| TDR | 26,769,378 | 26,769,397 | CTCCATCTCCAACGCACCAG |
PCR Primers
| Primer | Sequence |
| SU | TGTCACCACAACTGCCATAG |
| SD | TAGGCAGAGGTCCAAAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
