10521 Tmed6 Status: Cancelled
| Gene | Tmed6 |
| Chromosome | 8 |
| Deletion Start | 107,065,608 |
| Deletion End | 107,061,571 |
| Deletion Size | 4,038 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,065,575 | 107,065,593 | GTGGCTGAGCTGGTAGTTC |
| TUP | 107,065,548 | 107,065,574 | TGAGCCTCGTGACTTCTGTTAAGAGCC |
| TUR | 107,065,515 | 107,065,533 | GGTCCTTGGAGCCGTGTAG |
| TDF | 107,061,898 | 107,061,920 | TGTAGCACTGTTTCTCAGACTCC |
| TDP | 107,061,868 | 107,061,895 | AGTCACAGGTCACTTAGATCAGGGCAGG |
| TDR | 107,061,842 | 107,061,860 | GCCACTCTGGCTGCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGACCCTGCACTGCACCAACGG |
| SD | ACCCGGCAAGTTCTAGTAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 330 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 310 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
