10522 Oog4 Status: ES cell colonies screened / QC no positives
| Gene | Oog4 |
| Chromosome | 4 |
| Deletion Start | 143,440,013 |
| Deletion End | 143,437,451 |
| Deletion Size | 2,563 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,439,867 | 143,439,889 | AGGCAAGATCAGCTAGGGTAAAG |
| TUP | 143,439,836 | 143,439,860 | TCTCTGGGTGCTGAGAAGGAGCAAC |
| TUR | 143,439,772 | 143,439,794 | TCCCTCTAAGCTCATGCAATCAG |
| TDF | 143,437,944 | 143,437,962 | TGCCCTGTTGCCAGAATAC |
| TDP | 143,437,917 | 143,437,942 | TCTGCACTCAAACGTCTGAACCTGAC |
| TDR | 143,437,871 | 143,437,894 | GAGAACTCCAAGAGGATGAAAGAC |
PCR Primers
| Primer | Sequence |
| SU | GGCTCCCTAAATTTCTTGTC |
| SD | GGACAATCTTCTCATAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 769823 bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
