10525 Reg3b Status: Cancelled
| Gene | Reg3b |
| Chromosome | 6 |
| Deletion Start | 78,371,208 |
| Deletion End | 78,373,266 |
| Deletion Size | 2,059 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,372,106 | 78,372,125 | CCTGGAGGACACCTCGTATC |
| TUP | 78,372,126 | 78,372,151 | TGTGCTCAATAGCGCTGAGGCTTCAT |
| TUR | 78,372,168 | 78,372,191 | TGGTAGCTGTTTCCTGTTCTCTTC |
| TDF | 78,372,772 | 78,372,793 | GCTTTGGACCCTTCCTTTCATC |
| TDP | 78,372,794 | 78,372,819 | TGCATACTTCCTCACTAGGGTGCAGA |
| TDR | 78,372,828 | 78,372,850 | GTTACTCCATTCCCATCCACCTC |
PCR Primers
| Primer | Sequence |
| SU | TGTGTCCTCAGACAACACAG |
| SD | TAGAATGCAAAAACTCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
