10526 Derl3 Status: Cancelled
| Gene | Derl3 |
| Chromosome | 10 |
| Deletion Start | 75,893,409 |
| Deletion End | 75,895,319 |
| Deletion Size | 1,911 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,894,174 | 75,894,195 | CCCAGCTAGTTCTTCAGTGTCC |
| TUP | 75,894,197 | 75,894,224 | TGAACAGCACAACAGGGAGATATAGGGC |
| TUR | 75,894,261 | 75,894,280 | CCTGCTCCTTCTGGCTCTTG |
| TDF | 75,894,920 | 75,894,940 | TGTCTTTCAAGGCCAGTTCTG |
| TDP | 75,894,949 | 75,894,974 | CTGCCAGATAGCAGGGTACTTGGGCT |
| TDR | 75,894,987 | 75,895,007 | TCTTTCACAGCCCAGGGTAAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTAGATGCTGTCAAACG |
| SD | GGGGTGGCATCAGCCCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 378 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
