10527 2010002M12Rik Status: Cancelled
| Gene | 2010002M12Rik |
| Chromosome | 19 |
| Deletion Start | 34,629,756 |
| Deletion End | 34,618,780 |
| Deletion Size | 10,977 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,629,418 | 34,629,441 | AACCTTAATGGACAACCCAACCAC |
| TUP | 34,629,391 | 34,629,414 | TAATGCAAAGGCCACCTTGCTGGA |
| TUR | 34,629,356 | 34,629,379 | GCAAAGTAGGTAGGTCTCCATGTG |
| TDF | 34,618,943 | 34,618,962 | AACTGGCTGAAGGACGCAAG |
| TDP | 34,618,912 | 34,618,937 | TCATGTCCTGGAAAGCTTGAGCCTCC |
| TDR | 34,618,843 | 34,618,863 | GCCTCAGGGCCTTCTCATAAC |
PCR Primers
| Primer | Sequence |
| SU | CGGTATACTGGTACACCTGC |
| SD | GATGCAATCCAAAGGCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | 301 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
