10528 Mfsd6l Status: Cancelled
| Gene | Mfsd6l |
| Chromosome | 11 |
| Deletion Start | 68,556,309 |
| Deletion End | 68,558,086 |
| Deletion Size | 1,778 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,557,040 | 68,557,061 | CACCGTGGACTTTCATCCTCTC |
| TUP | 68,557,070 | 68,557,097 | TTGTTGTATTCTGGGAACTCCTGGCAGC |
| TUR | 68,557,108 | 68,557,126 | GACTGTCATCCGCCACCTG |
| TDF | 68,557,310 | 68,557,328 | CCCTGGCCTTAGCAGTGAG |
| TDP | 68,557,329 | 68,557,355 | CACTGCCTTTCCTGTTCCCATAGACCA |
| TDR | 68,557,379 | 68,557,400 | ATGAGGGACAGAGCTTTGATGG |
PCR Primers
| Primer | Sequence |
| SU | GCAAATAGCCGTTGGCTAGG |
| SD | ACCCACATATGTATACAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 270 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 277 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
