10531 6330527O06Rik Status: Cancelled
| Gene | 6330527O06Rik |
| Chromosome | 2 |
| Deletion Start | 136,058,298 |
| Deletion End | 136,069,197 |
| Deletion Size | 10,900 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,058,813 | 136,058,832 | ATGGCTTGCGGAGCACACTG |
| TUP | 136,058,834 | 136,058,858 | TTCGAGCAGGGAACTTTGCCTCTCC |
| TUR | 136,058,883 | 136,058,902 | AGCCCTCTGCTTTAGGACAC |
| TDF | 136,069,032 | 136,069,053 | TGAGCAAGAGCAGTTGGAAGAG |
| TDP | 136,069,061 | 136,069,085 | CCCTGATCCTGGGCCTCATCTTGGG |
| TDR | 136,069,105 | 136,069,127 | TGGTGGATGTGGTAGATCACAAG |
PCR Primers
| Primer | Sequence |
| SU | ATCCTGGGTGCTTCCTCTG |
| SD | GCAGTATGGACACAACATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 306 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 297 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
