10532 Myl3 Status: Cancelled
| Gene | Myl3 |
| Chromosome | 9 |
| Deletion Start | 110,763,727 |
| Deletion End | 110,769,443 |
| Deletion Size | 5,717 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,763,865 | 110,763,886 | CCCAAGGAAGCCGAGTTTGATG |
| TUP | 110,763,887 | 110,763,913 | CCTCCAAGATTAAGGTGAGCGGGTAGC |
| TUR | 110,763,918 | 110,763,937 | TGGCTCTGTGCCTTCTACAC |
| TDF | 110,768,822 | 110,768,842 | GCCGACTTCAAAGGGCTACTC |
| TDP | 110,768,843 | 110,768,868 | CAAGAGACTGCTACCAGAAGGCAGGG |
| TDR | 110,768,869 | 110,768,891 | TCGTGCATATACTGTCCTCTGTC |
PCR Primers
| Primer | Sequence |
| SU | GAATGGAGCACACTCCCCT |
| SD | GAGTCCGAACCACTCCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 357 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
