10533 Rpl13 Status: Cancelled
| Gene | Rpl13 |
| Chromosome | 8 |
| Deletion Start | 123,102,645 |
| Deletion End | 123,105,202 |
| Deletion Size | 2,558 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,103,291 | 123,103,311 | GAGTACCGCTCCAAGCTCATC |
| TUP | 123,103,318 | 123,103,339 | CCCAGGAAGCCTTCTGCTCCGA |
| TUR | 123,103,352 | 123,103,370 | CCCGCAGGACTTACAGAAC |
| TDF | 123,104,639 | 123,104,657 | GCCTTTGCTGTCCTGGAAC |
| TDP | 123,104,659 | 123,104,680 | CAGTACACCAACCAGGCTGGCC |
| TDR | 123,104,682 | 123,104,703 | AGCAGATGCAGGTGTGGGTTAG |
PCR Primers
| Primer | Sequence |
| SU | GTCCCATCGTGGTCCATCG |
| SD | CGACCACCCCAAGGAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 311 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 347 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
