10538 5730409E04Rik Status: Cancelled
| Gene | 5730409E04Rik |
| Chromosome | 4 |
| Deletion Start | 126,611,684 |
| Deletion End | 126,612,645 |
| Deletion Size | 962 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,611,752 | 126,611,771 | CCAACCAGACCTCCAACCAG |
| TUP | 126,611,780 | 126,611,808 | TCCAACTTTGTGGAGAGTGCTAAGGATGC |
| TUR | 126,611,809 | 126,611,831 | GCACACCATGCCAGTTCTTATTG |
| TDF | 126,612,301 | 126,612,322 | GGACAACCGGAAGGAGCTTATC |
| TDP | 126,612,326 | 126,612,348 | TGTCTGCAACAGAGGGAAGCACC |
| TDR | 126,612,362 | 126,612,380 | CACACCCTTAGCCCTGGTC |
PCR Primers
| Primer | Sequence |
| SU | GGTTGAGTAGGGGTTGAATC |
| SD | GAACAGGCCACAGCCCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 400 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 359 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
