| Gene | Ifit1 |
| Chromosome | 19 |
| Deletion Start | 34,640,967 |
| Deletion End | 34,648,857 |
| Deletion Size | 7,891 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,641,281 | 34,641,305 | ACAGGCCCTCTGGAATGGAATAAAG |
| TUP | 34,641,310 | 34,641,332 | ACCAGTGCCCACTTGGTGCTCCA |
| TUR | 34,641,353 | 34,641,375 | TCAGAAGATCAGAAGGAGCATAC |
| TDF | 34,648,364 | 34,648,384 | AGCCCAGAAGGCAGGACAATG |
| TDP | 34,648,385 | 34,648,408 | TGCAAGAATTGGCACAACAGGCCA |
| TDR | 34,648,450 | 34,648,473 | AGCCATGCAAACATAGGCCATCTC |
PCR Primers
| Primer | Sequence |
| SU | AGTGCCCCACAGTGCGTCTC |
| SD | AGACATAAGGAAAAAGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 357 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 367 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10542A-H6 | VGB6 | C57BL/6NTac | |
| 10542B-B6 | VGB6 | C57BL/6NTac | |
| 10542B-D12 | VGB6 | C57BL/6NTac | |
| 10542B-H8 | VGB6 | C57BL/6NTac | |
| 10542B-D8 | VGB6 | C57BL/6NTac | |
