10545 Cd300ld Status: ES cell colonies screened / QC one positive
| Gene | Cd300ld |
| Chromosome | 11 |
| Deletion Start | 114,989,807 |
| Deletion End | 114,983,750 |
| Deletion Size | 6,058 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,989,544 | 114,989,564 | ACCTTCCAACCCTAGCTGCTC |
| TUP | 114,989,516 | 114,989,543 | CACGAAAGCCGATGAGTGAGTGGATGAA |
| TUR | 114,989,464 | 114,989,483 | CTAGGCTGCATGTGCCAAAC |
| TDF | 114,984,723 | 114,984,742 | GACAGAGTACAGCAGCCATC |
| TDP | 114,983,464 | 114,983,486 | CGGGCCACCAGCCAGTTGATTGA |
| TDR | 114,984,659 | 114,984,679 | GCTACTTGGTGAGGCAACATC |
PCR Primers
| Primer | Sequence |
| SU | ATCTCTCCTCCTGATTCC |
| SD | CTGGCTGGTGGCCCGCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 512 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 1053 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
